Ohmic heating as a new tool for protein scaffold engineering

Ohmic heating (OH) is recognised as an emerging processing technology which recently gaining increasing attention due to its ability induce and control protein functionality. In this study, OH was used for the first time in production of scaffolds tissue engineering. BSA/casein solutions were processed by OH, promoting denaturation aggregation, followed cold-gelation through addition Ca2+. The formation stable mostly dependent on temperature treatment during processing. variations electric field (EF) induced changes functional properties both gel forming final (contact angle, swelling, porosity, compressive modulus degradation rate). scaffolds' biological performance evaluated regarding their support adhesion proliferation human fibroblast cells. process resulted a non-cytotoxic material imposed presence EF improved cellular metabolic activity. Protein functionalization assisted presents promising new alternative tuneable protein-based